FC
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and HeLa cells (treated with UV for 30min,right) were intracellularly-stained with PE Rabbit anti Human Phospho-Histone H2AX-S139 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).FC
Flow cytometry: 1X10^6 NIH/3T3 cells (negative control,left) and NIH/3T3 cells (treated with UV for 30min,right) were intracellularly-stained with PE Rabbit anti Human Phospho-Histone H2AX-S139 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).| Product Name | PE Rabbit anti-Human Phospho-Histone H2AX-S139 mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S319 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FC |
| FC:5 μl per 10^6 cells in 100 μl volume |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Alternative Names | H2A.X H2A/X H2AFX |
| Cellular Localization | Chromosome, Nucleus. |
FC
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and HeLa cells (treated with UV for 30min,right) were intracellularly-stained with PE Rabbit anti Human Phospho-Histone H2AX-S139 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
FC
Flow cytometry: 1X10^6 NIH/3T3 cells (negative control,left) and NIH/3T3 cells (treated with UV for 30min,right) were intracellularly-stained with PE Rabbit anti Human Phospho-Histone H2AX-S139 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).| Application Notes | FC:5 μl per 10^6 cells in 100 μl volume |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8℃. Avoid freeze. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3. |
