All products Antibodies Anti-Smad5 antibody

Anti-Smad5 antibody

Catalog Number：OM642143

  • Western blot analysis using Smad5 antibody against A431(1), COS-7(2) cell lysate.

  • Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissues using Smad5 antibody with DAB staining.

  • Immunofluorescence analysis of MG-63 cells using Smad5 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Product Profile

Product Name Anti-Smad5 antibody
Antibody Type Primary Antibodies
Immunogen 
Polypeptide

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol SMAD5
Gene Synonyms
DWFC
JV5-1
MADH5
Gene Full Name SMAD family member 5
Gene Summary
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Molecular Weight(MW) 52 kDa
Source Rabbit
Cellular Localization Cytoplasm, Nucleus

Application

  • WB

    Western blot analysis using Smad5 antibody against A431(1), COS-7(2) cell lysate.

  • IHC

    Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissues using Smad5 antibody with DAB staining.

  • ICC/IF

    Immunofluorescence analysis of MG-63 cells using Smad5 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Application Notes
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500

Additional Information

Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM642143
  • Price: $336.00
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  • Lead time: 4~6 Week

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