SMAD5 Antibody

Product Profile

Product Name	SMAD5 Antibody
Antibody Type	Primary Antibodies
Product description	SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.1) Langenfeld, E.M., et al., (2006) Oncogene 25 (5), 685-692.
Immunogen	Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
Tested Applications	WB
	SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity	DogMouseRat
Concentration	1 mg/ml
Purification	Protein A

Target Information

Gene Symbol	SMAD5
Alternative Names	SMAD5 DWFC JV5-1 MADH5
Molecular Weight(MW)	51 kDa, 52 kDa, 52 kDa

Database Links

Entrez Gene	4090
Protein Accession	NP_001001420

Application

Positive Control	1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes	SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form	Liquid
Storage Instructions	For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer	IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt

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• Data sheet for OM291670