Antibody used in WB on r-SMAD5 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.1) Zimonjic, D.B., et al., (2003) Neoplasia 5 (5), 390-396.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on r-SMAD5 at: 1:1000 (Lane 1: 5ug of transfected 293T lysate (SMAD1) , Lane 2: 5ug of transfected 293T lysate (SMAD2) , Lane 3: 5ug of transfected 293T lysate (SMAD3) , Lane 4: 5ug of transfected 293T lysate (SMAD4) , Lane 5: 5ug of transfected 293T lysate (SMAD5) , Lane 6: 5ug of transfected 293T lysate (SMAD6) , Lane 7: 5ug of transfected 293T lysate (SMAD7) , Lane 8: 5ug of
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
SMAD5 antibody can be used for detection of SMAD5 by ELISA at 1:62500. SMAD5 antibody can be used for detection of SMAD5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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