Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CCNA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of CCNA2 using rabbit polyclonal CCNA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCNA2 gene (Lane 2).
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.1) Henglein B.,Proc. Natl. Acad. Sci. U.S.A. 91:5490-5494(1994)
Immunogen
This CCNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-389 amino acids from the C-terminal region of human CCNA2.
Key Feature
Clonality
Polyclonal
Isotype
Ig
Host Species
Rabbit
Tested Applications
IHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CCNA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CCNA2 using rabbit polyclonal CCNA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCNA2 gene (Lane 2).
Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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