All products Antibodies Cyclin A Antibody

Cyclin A Antibody

Catalog Number：OM276522

  • Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Western blot analysis of anti-CCNA2Pab in K562 cell line lysates (35ug/lane).

Product Profile

Product Name Cyclin A Antibody
Antibody Type Primary Antibodies
Product description 
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.1) Henglein B.,Proc. Natl. Acad. Sci. U.S.A. 91:5490-5494(1994)
Immunogen 
This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol CCNA2
Alternative Names
Cyclin-A2
Cyclin-A
CCNA2
CCN1
CCNA
Molecular Weight(MW) 49 kDa

Database Links

Entrez Gene 890
Protein Accession P20248

Application

  • Application

    Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western blot analysis of anti-CCNA2Pab in K562 cell line lysates (35ug/lane).

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM276522
  • Price: $336.00
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