All products Antibodies IPF Antibody

IPF Antibody

Catalog Number:OM282245

  • Western blot analysis in U-937 cell line lysates (35ug/lane).

  • Fluorescent confocal image of U251 cell stained with IPF Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7

Product Profile

Product Name IPF Antibody
Antibody Type Primary Antibodies
Product description
IPF is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. This nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).1) Ma,J., Carcinogenesis 29 (7), 1327-1333 (2008)
Immunogen
This IPF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human IPF.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB

For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1 mg/ml

Target Information

Gene Symbol PDX1
Alternative Names
Pancreas/duodenum homeobox protein 1
PDX-1
Glucose-sensitive factor
GSF
Insulin promoter factor 1
IPF-1
Insulin upstream factor 1
IUF-1
Islet/duodenum homeobox-1
IDX-1
Somatostatin-transactivating factor 1
STF-1
PDX1
IPF1
STF1
Molecular Weight(MW) 31 kDa

Database Links

Entrez Gene 3651
Protein Accession P52945

Application

  • Application

    Western blot analysis in U-937 cell line lysates (35ug/lane).

  • Application

    Fluorescent confocal image of U251 cell stained with IPF Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IPF primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM282245
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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