IRF3 is interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.1) Korherr, C., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (11), 4240-4245.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IRF3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
IRF3 antibody can be used for detection of IRF3 by ELISA at 1:312500. IRF3 antibody can be used for detection of IRF3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 5.0 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
IRF3 antibody can be used for detection of IRF3 by ELISA at 1:312500. IRF3 antibody can be used for detection of IRF3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store IRF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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