All products Antibodies STAT3 (phospho Ser727) Antibody

STAT3 (phospho Ser727) Antibody

Catalog Number：OM292337

  • Western blot analysis of lysed extracts from HeLa and HepG2 cells untreated or treated with PMA using STAT3 (Phospho-Ser727).

  • Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Phospho-Ser727).

Product Profile

Product Name STAT3 (phospho Ser727) Antibody
Antibody Type Primary Antibodies
Product description 
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.1) Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804. Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115. Garcia R, et al. (1998) J Biomed Sci. 5: 79-85. Bromberg J F, et al. (1999) Cell. 98: 295-303.
Immunogen 
STAT3 (Phospho-Ser727) antibody was raised against a peptide sequence around phosphorylation site of serine 727 (P-M-S (p) -P-R) derived from Human STAT3.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IFIHCWB
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity

HumanMouseRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol STAT3
Alternative Names
APRF
HIES
STAT5
APRF
Acute-phase response factor
Molecular Weight(MW) 88 kDa
Tissue Specificity This antibody detects endogenous level of STAT3 only when phosphorylated at serine 727.

Database Links

Entrez Gene 6774
Protein Accession NP_003141.2

Application

  • Application

    Western blot analysis of lysed extracts from HeLa and HepG2 cells untreated or treated with PMA using STAT3 (Phospho-Ser727).

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Ser727). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using STAT3 (Phospho-Ser727).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
Storage Instructions Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM292337
  • Price: $336.00
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