TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process.1) Zakut-Houri, R., et al., (1985) EMBO J. 4: 1251-1255..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TP53.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
TP53 antibody can be used for detection of TP53 by ELISA at 1:62500. TP53 antibody can be used for detection of TP53 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human DU145 cells at 0.5 ug/ml.
Application Notes
TP53 antibody can be used for detection of TP53 by ELISA at 1:62500. TP53 antibody can be used for detection of TP53 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TP53 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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