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p53 Antibody

Catalog Number：OM287197

  • Western blot analysis in 293 cell line lysates (35ug/lane).

  • Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific). Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

  • Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

Product Profile

Product Name p53 Antibody
Antibody Type Primary Antibodies
Product description 
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.1) Chen L, et al.  Oncogene. 2003 Aug 14;22(34):5348-57.
Immunogen 
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IHC-PWB
For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:100:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol TP53
Alternative Names
Cellular tumor antigen p53
Antigen NY-CO-13
Phosphoprotein p53
Tumor suppressor p53
TP53
P53
Molecular Weight(MW) 44 kDa

Database Links

Entrez Gene 7157
Protein Accession P04637

Application

  • Application

    Western blot analysis in 293 cell line lysates (35ug/lane).

  • Application

    Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific). Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

  • Application

    Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes
For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:100:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM287197
  • Price: $336.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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