Application
Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | AFP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HPAFP |
|---|---|
| Alternative Names | AFP HPAFP Alpha-fetoprotein Alpha-1-fetoprotein Alpha-fetoglobulin |
| Molecular Weight(MW) | 68678 Da |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties |
| Tissue Specificity | Plasma. Synthesized by the fetal liver and yolk sac |
| Cellular Localization | Secreted. |
| Entrez Gene | 174 |
|---|
Application
Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
