Application
Dot blot analysis of anti-hH4-K20[Me3] methylation-specific Pab on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
H4-K20[Me3] western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).Application
H4-K20[Me3] western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).| Product Name | H4 Antibody ( K20 ) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIST1H4A; H4/A; H4FA; Histone H4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| DB:1:500 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H4/A H4FA |
|---|---|
| Alternative Names | HIST1H4A H4/A H4FA Histone H4 |
| Molecular Weight(MW) | 11367 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic methylation-peptide between 4~33 amino acids Me3 of human hH4-K20[Me3]. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8370 |
|---|
Application
Dot blot analysis of anti-hH4-K20[Me3] methylation-specific Pab on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
H4-K20[Me3] western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).
Application
H4-K20[Me3] western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).| Application Notes | DB:1:500 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step methylation specific peptide affinity purification. |
