Application
Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab (Cat. AP3674a) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
H4 Antibody ( K20 [Me2] ) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | H4 Antibody ( K20 ) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HIST1H4A; H4/A; H4FA; Histone H4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIHCFC |
| DB:1:500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H4/A H4FA |
|---|---|
| Alternative Names | HIST1H4A H4/A H4FA Histone H4 |
| Molecular Weight(MW) | 11367 Da |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling |
| Tissue Specificity | This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic methylation peptide between 4~33 amino acids surrounding K20(Methyl 2) of human H4. |
| Cellular Localization | Nucleus. Chromosome. |
| Entrez Gene | 8370 |
|---|
Application
Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab (Cat. AP3674a) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
H4 Antibody ( K20 [Me2] ) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | DB:1:500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step methylation specific peptide affinity purification. |
