Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000.| Product Name | APE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse polyclonal to APE1Apurinic / Apyrimidinic Endonuclease (APE 1 or REF 1) is responsible for the incision of DNA at abasicsites during base excision repair. It has also been shown to stimulate the sequence-specific DNA binding activity of Fos-Jun (AP 1) heterodimers. Alterations in APE 1 levels have been described insome cancers. |
| Immunogen | Full length human APE1 purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: 1:500-1:3000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 328, APEX, REF1, 107748, P27695, APE1, APEX1, HAP1, APE, APX, REF-1, APEN, REF 1 |
|---|---|
| Target | APE1 |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | For WB: 1:500-1:3000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Antiserum containing no preservatives |
