Application
at a 1/1000 dilution staining ~ 38 kDa APE 1 in 35mg of: A) HepG2 whole cell lysate; B) HeLa whole cell lysate; C) Raji whole cell lysate.| Product Name | APE1 antibody [2104] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2104] to APE1Apurinic / Apyrimidinic Endonuclease (APE 1 or REF 1) is responsible for the incision of DNA at abasicsites during base excision repair. It has also been shown to stimulate the sequence-specific DNA binding activity of Fos-Jun (AP 1) heterodimers. Alterations in APE 1 levels have been described insome cancers. |
| Immunogen | Full length human APE1 purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 2104 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| For ELISA: Use at a concentration of 1:1,000 - 1:10,000. For IHC-P: Use at a dilution of 1:5,000. For WB: Use at a dilution of 1:1,000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 328, APEX, REF1, 107748, P27695, APE1, APEX1, HAP1, APE, APX, REF-1, APEN, REF 1 |
|---|---|
| Target | APE1 |
Application
at a 1/1000 dilution staining ~ 38 kDa APE 1 in 35mg of: A) HepG2 whole cell lysate; B) HeLa whole cell lysate; C) Raji whole cell lysate.| Positive Control | HepG2, HeLa, Raji whole cell lysates |
|---|---|
| Application Notes | For ELISA: Use at a concentration of 1:1,000 - 1:10,000. For IHC-P: Use at a dilution of 1:5,000. For WB: Use at a dilution of 1:1,000. Predicted molecular weight: 40 kDa. Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, no preservatives added |
