Application
Western Blot: APE1 Antibody (13B8E5C2) - Ovarian Cancer cell lines.Application
Immunocytochemistry/Immunofluorescence: APE1 Antibody (13B8E5C2) - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDS231.Application
Immunohistochemistry-Paraffin: APE1 Antibody (13B8E5C2) - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.Application
Immunocytochemistry/Immunofluorescence: APE1 Antibody (13B8E5C2) - HeLa cells stained (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).| Product Name | APE1 antibody [13B8E5C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [13B8E5C2] to APE1Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NFkB, Myb, HIF1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. Altered levels of APE have also been found in some cancers. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 13B8E5C2 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| ICC: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/5000. IHC-Fr: Use at a dilution of 1/5000. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/2000. Predicted molecular weight: 37 kDa. Can be competed from recognizing APE/ref-1 antigen in tissues using APE/ref-1 protein. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 328, APEX, REF1, 107748, P27695, APE1, APEX1, HAP1, APE, APX, REF-1, APEN, REF 1 |
|---|---|
| Target | APE1 |
| Tissue Specificity | This antibody is specific to the human APE/ref-1 protein. |
| Cellular Localization | Nuclear |
Application
Western Blot: APE1 Antibody (13B8E5C2) - Ovarian Cancer cell lines.
Application
Immunocytochemistry/Immunofluorescence: APE1 Antibody (13B8E5C2) - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDS231.
Application
Immunohistochemistry-Paraffin: APE1 Antibody (13B8E5C2) - APE1 antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain.
Application
Immunocytochemistry/Immunofluorescence: APE1 Antibody (13B8E5C2) - HeLa cells stained (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).| Application Notes | ICC: Use at an assay dependant dilution. IHC-P: Use at a dilution of 1/5000. IHC-Fr: Use at a dilution of 1/5000. IP: Use at an assay dependant dilution. WB: Use at a dilution of 1/2000. Predicted molecular weight: 37 kDa. Can be competed from recognizing APE/ref-1 antigen in tissues using APE/ref-1 protein. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS and Ascitic fluid. |
