Application
Extract of 293T cells transfected with ARTS plasmid was separated on SDS-PAGE and probed with Monoclonal PA to ARTS (MO) CL:ART S51 . The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody dilution 1/4,000.| Product Name | ARTS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [ARTS51] to ARTSApoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF beta and, to a lesser extent, by other apoptotic agents, such as TNF alpha and Fas ligand. |
| Immunogen | Synthetic peptide: NIQDNRVHCCLYFI SPFGHG conjugated to KLH, corresponding to amino acids 247-266 of Human ARTS. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | ARTS51 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a dilution of 1/0.5 - 1/1.0. Detects a band of approximately 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | hCDCREL2 603696 H5 hCDCREL-2 hucep-7 5414 ARTS O43236 hucep7 SEPT4 PNUTL2 CE5B3 hucep 7 hCDCREL 2 |
|---|---|
| Target | ARTS |
| Cellular Localization | Mitochondrial and Nuclear |
Application
Extract of 293T cells transfected with ARTS plasmid was separated on SDS-PAGE and probed with Monoclonal PA to ARTS (MO) CL:ART S51 . The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate. Antibody dilution 1/4,000.| Positive Control | Whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS. |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a dilution of 1/0.5 - 1/1.0. Detects a band of approximately 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
