Application
WB ImageHuman Raji Burkitt’s lymphoma cell extract was separated on SDS-PAGE and probed with Monoclonal PA to BCL-10 CL:151 . The antibody was developed with Biotin APA Mouse Fab ad/HuBov, Hor, Alkaline Phosphatase ExtrAvidin and an NBT/BCIP substrate.| Product Name | BCL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [151] to BCL10Bcl 10, also designated as CIPER, mE10, cE10, CARMEN, and CLAP, is an N-terminal CARD (Caspase Recruitment Domain) containing protein. It is a cellular homologue of the equine herpesvirus-2 protein E-10 (vCLAP). Bcl 10 was implicated in the regulation of apoptosis by interacting with caspase 9, enhancing procaspase 9 processing, and triggering its activation when overexpressed in the cell. Bcl 10 cellular overexpression induces JNK, p38, and NF-kappaB activation. Deregulation of Bcl 10 expression was also demonstrated to be involved in cellular oncogenesis. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 151 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: Use at a concentration of 1 - 2 μg/ml. Detects bands of approximately 20, 32 and 37 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CARMEN mE10 cE10 CLAP 603517 CIPER c-E10 8915 Bcl10 O95999 c E10 |
|---|---|
| Target | BCL10 |
| Cellular Localization | Cytoplasmic |
Application
WB ImageHuman Raji Burkitt’s lymphoma cell extract was separated on SDS-PAGE and probed with Monoclonal PA to BCL-10 CL:151 . The antibody was developed with Biotin APA Mouse Fab ad/HuBov, Hor, Alkaline Phosphatase ExtrAvidin and an NBT/BCIP substrate.| Positive Control | Raji whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: Use at a concentration of 1 - 2 μg/ml. Detects bands of approximately 20, 32 and 37 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
