Application
Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).Application
Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).Application
Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.Application
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.Application
Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dyeApplication
Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Product Name | CD44 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8E2F3] to CD44The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 628 and 699 of human CD44 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 8E2F3 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Synonyms | IN MDU2 CSPG8 HUTCH-I CDW44 LHR MIC4 ECMR-III MDU3 HCELL PGP1 MC56 |
|---|---|
| Target | CD44 |
| Molecular Weight(MW) | 82 kDa (note) |
Application
Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).
Application
Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Application
Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.
Application
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Application
Confocal immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin Blue: DRAQ5 fluorescent DNA dye
Application
Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
