Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-GLULmouse monoclonal antibody. ( Dilution 1:50)Application
Immunofluorescent staining of HepG2 cells using anti-GLUL mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-GLUL mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-GLULmouse monoclonal antibody. ( Dilution 1:50)Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GLUL monoclonal antibody.Application
Flow cytometric analysis of Hela cells, using anti-GLUL antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Flow cytometric analysis of Jurkat cells, using anti-GLUL antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).Application
Anti-GLUL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GLULApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLUL.| Product Name | Glutamine synthetase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F4] to GLULThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. |
| Immunogen | Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 1F4 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GLNS GS GLUL |
|---|---|
| Target | GLUL |
| Tissue Specificity | is specific for human GLUL (NP_002056). |
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-GLULmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunofluorescent staining of HepG2 cells using anti-GLUL mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded breast tissue using anti-GLUL mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-GLULmouse monoclonal antibody. ( Dilution 1:50)
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GLUL monoclonal antibody.
Application
Flow cytometric analysis of Hela cells, using anti-GLUL antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Flow cytometric analysis of Jurkat cells, using anti-GLUL antibody,(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Application
Anti-GLUL mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GLUL
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLUL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLUL.| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
