| Product Name | Her2 / ErbB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [N24] to ErbB2ErbB2 exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. This antibody reacts with the intact cells, slightly increases (~2-fold) the tyrosine kinase activity, accelerates (~50%) c-erbB-2 turnover, and inhibits (~80%) tumorigenic growth in CD1/nude mice of N87 human gastric carcinoma cell line overexpressng ErbB2. |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with intact SKBR-3 breast cancer cells and mouse myeloma cells |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | N24 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIP |
| For IF: Use at an assay dependent dilution. For IP (Protein G): Use at 2μg/mg of lysate. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | c-erb B2 HER2 137215 cerb B2 ErbB2 NGL HER2/neu P04626 2064 NEU HER-2/neu HER-2 TKR1 CD340 MLN 19 HER 2 c erb B2 HER 2/neu |
|---|---|
| Target | ErbB2 |
| Cellular Localization | Cell Membrane |
| Positive Control | SKBR-3 cells or breast carcinomas |
|---|---|
| Application Notes | For IF: Use at an assay dependent dilution. For IP (Protein G): Use at 2μg/mg of lysate. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 10mM Phosphate-buffered saline, pH 7.4, containing 0.2% BSA and 0.09% sodium azide |
