Application
Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT282] . Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT282] . EasyBlot anti-mouse IgG was used as a secondary reagent.Application
Histone H4 (di methyl Lys20) antibody [GT282] detects Histone H4 (di methyl Lys20) protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [GT282] dilution: 1:5000Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.Application
Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H4K20me2 (dimethyl Lys20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT282] to Histone H4 (di methyl Lys20) (histone cluster 1, H4a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Modification | dimethyl Lys20 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT282 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (100%), Xenopus laevis (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Histone H4 di-Methyl Lys20 di-Methyl K20 Histone H4 di Methyl K20 Histone H4 H4FA Histone H4 di-Methyl Histone H4 di Methyl di-Methyl Lys20 Histone H4 di Methyl Histone H4 Lys20 Antibod |
|---|---|
| Target | Histone H4 (di-Methyl Lys20) |
| Molecular Weight(MW) | 11 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT282] . Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT282] . EasyBlot anti-mouse IgG was used as a secondary reagent.
Application
Histone H4 (di methyl Lys20) antibody [GT282] detects Histone H4 (di methyl Lys20) protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa nuclear lysate/extract Histone H4 (di methyl Lys20) antibody [GT282] dilution: 1:5000
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.
Application
Histone H4 (di-Methyl Lys20) antibody [GT282] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT282] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
