Application
Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector. Lane 2: M8 cell line transfected with HLA-G1. Lane 3: M8 cell line transfected with HLA-G5. Lane 4: M8 cell line transfected with HLA-G6| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [5A6G7] to HLA-GHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A6G7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-FIHC-PWB |
| WB: Positive Control: JEG-3 cell lysate. Note: reducing conditions, 12% AA SDS-PAGE: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HLA G HLAG |
|---|---|
| Target | HLA-G |
| Tissue Specificity | The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
Application
Western Blotting analysis of whole cell lysate of HLA-G stable transfectants (various splice variants) using anti-human HLA-G (5A6G7). Lane 1: M8 cell line transfected with empty vector. Lane 2: M8 cell line transfected with HLA-G1. Lane 3: M8 cell line transfected with HLA-G5. Lane 4: M8 cell line transfected with HLA-G6| Application Notes | WB: Positive Control: JEG-3 cell lysate. Note: reducing conditions, 12% AA SDS-PAGE: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
