Application
PDE4B antibody detects PDE4B protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE4B protein stained by PDE4B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PDE4B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PDE4B (phosphodiesterase 4B, cAMP-specific)This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDE4B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (82%), Rat (82%), Pig (97%), Rhesus Monkey (85%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | phosphodiesterase 4B cAMP-specific DPDE4 PDE4B5 PDEIVB |
|---|---|
| Target | PDE4B |
| Molecular Weight(MW) | 83 kDa (note) |
Application
PDE4B antibody detects PDE4B protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PDE4B protein stained by PDE4B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
