Application
Western blot analysis of extracts of various cell lines, using EGLN1 antibody.Application
Immunohistochemistry of paraffin-embedded human kidney using EGLN1 antibody at dilution of 1:200 (x400 lens)Application
Immunofluorescence analysis of MCF-7 cell using EGLN1 antibody. Blue: DAPI for nuclear staining.| Product Name | PHD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PHD2The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq, Nov 2009] |
| Immunogen | Recombinant protein of human EGLN1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EGLN1 C1orf12 HIF PH2 ZMYND6 HPH2 HIFPH2 ECYT3 egl-9 family hypoxia-inducible factor 1 SM20 |
|---|---|
| Target | PHD2 |
Application
Western blot analysis of extracts of various cell lines, using EGLN1 antibody.
Application
Immunohistochemistry of paraffin-embedded human kidney using EGLN1 antibody at dilution of 1:200 (x400 lens)
Application
Immunofluorescence analysis of MCF-7 cell using EGLN1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
