Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EGLN1 antibody diluted at 1:500.| Product Name | PHD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to EGLN1 (egl nine homolog 1 (C. elegans))The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human EGLN1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (94%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ZMYND6 egl nine homolog 1 (C. elegans) PHD2 HPH-2 ECYT3 C1orf12 SM20 HIF-PH2 HIFPH2 HPH2 |
|---|---|
| Target | EGLN1 |
| Molecular Weight(MW) | 46 kDa (note) |
Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with EGLN1 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
