WB
Western blot analysis of Phospho-Histone H2A.X (Ser139) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) antibody(upper) or beta Actin antibody.(lower)ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.| Product Name | Phospho-Histone H2A.X (Ser139) (2A9) Mouse mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Molecular Weight(MW) | 15kDa |
| Cellular Localization | Nucleus,Chromosome. |
WB
Western blot analysis of Phospho-Histone H2A.X (Ser139) in untreated or Hydroxyureatreated Hela and A549 lysates using Phospho-Histone H2A.X (Ser139) antibody(upper) or beta Actin antibody.(lower)
ICC/IF
Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.| Application Notes | WB:1:500-1:1000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Data sheet for OM643278