WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.WB
U-251 cells were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Parkin antibody at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L),CL594-Phalloidin (red).| Product Name | PARK2/Parkin Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Parkin protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:8000 IHC:1:250-1:1000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | PDJ AR-JP LPRS2 PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Alternative Names | PARK2 Parkin 2H5A7 E3 ubiquitin-protein ligase parkin EC:2.3.2.31 |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Cell projection,Cytoplasm,Endoplasmic reticulum,Membrane,Mitochondrion,Mitochondrion outer membrane,Nucleus,Synapse. |
WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB
mouse brain tissue were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB
U-251 cells were subjected to SDS PAGE followed by western blot with Parkin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Parkin antibody at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using PARK2/Parkin antibody at dilution of 1:400 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L),CL594-Phalloidin (red).| Application Notes | WB:1:2000-1:8000 IHC:1:250-1:1000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
