WB
Western blot analysis of GAD67 on different lysates with Mouse anti-GAD67 antibody at 1/10,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 (Mouse, Green), VGLUT1, 1:500 (Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 ( Mouse, Green), VGLUT1, 1:500 (Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.| Product Name | GAD67 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human GAD67 aa 1-594. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:10000 IHC:1:500-1:2000 IF-F:1:500 IF-P:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Gad1 |
|---|---|
| Gene Synonyms | EP10 Gad-1 |
| Gene Full Name | glutamate decarboxylase 1 |
| Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in three products, the predominant 67 kDa isoform, a smaller 25 kDa isoform, and an even smaller 49 aa isoform. The two shorter isoforms are not thought to retain catalytic activity. [provided by RefSeq, Apr 2023] |
| Alternative Names | 67 kDa glutamic acid decarboxylase antibody CPSQ1 antibody DCE1 antibody DCE1_HUMAN antibody EC 4.1.1.15 antibody FLJ45882 antibody GAD 67 antibody GAD antibody GAD-67 antibody GAD1 antibody 67 kDa glutamic acid decarboxylase antibody CPSQ1 antibody DCE1 antibody DCE1_HUMAN antibody EC 4.1.1.15 antibody FLJ45882 antibody GAD 67 antibody GAD antibody GAD-67 antibody GAD1 antibody Glutamate decarboxylase 1 (brain 67kDa) antibody Glutamate decarboxylase 1 antibody Glutamate decarboxylase 1 brain 67kD antibody Glutamate decarboxylase 1 brain 67kDa antibody Glutamate decarboxylase 67 kDa isoform antibody Glutamate decarboxylase brain 67-KD antibody OTTHUMP00000041055 antibody SCP antibody |
| Cellular Localization | Cytoplasm, Plasma Membrane. |
WB
Western blot analysis of GAD67 on different lysates with Mouse anti-GAD67 antibody at 1/10,000 dilution.Lysates/proteins at 20 µg/Lane.Exposure time: 6 seconds;4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-GAD67 antibody at 1/500 dilution. Important Notice: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (488) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).
IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 (Mouse, Green), VGLUT1, 1:500 (Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× PBST. Endogenous peroxidase blocking: 3% H2O2, 10 minutes. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: GAD67, 1/500 ( Mouse, Green), VGLUT1, 1:500 (Guinea pig, Red), overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), Goat anti-Guinea pig IgG (594), 1.5 hours at room temperature.| Application Notes | WB:1:10000 IHC:1:500-1:2000 IF-F:1:500 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
