ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptideDB
Dot blot analysis was performed using Anti-Phospho-CaMKII alpha/delta (Thr286) 277-288 antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Product Name | Anti-Phospho-CaMKII alpha/delta (Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide conjugated with KLH. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBELISA |
| DB:1:1000 |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK2A |
|---|---|
| Gene Synonyms | CAMKA MRD53 MRT63 CaMKIIalpha CaMKIINalpha |
| Gene Full Name | calcium/calmodulin dependent protein kinase II alpha |
| Gene Summary | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. |
| Alternative Names | Alpha CaMKII Calcium calmodulin dependent protein kinase II Calcium/calmodulin dependent protein kinase II alpha B subunit Calcium/calmodulin dependent protein kinase type II alpha chain Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha Calcium/calmodulin-dependent protein kinase II alpha Calcium/calmodulin-dependent protein kinase II-alpha Calcium/calmodulin-dependent protein kinase type II subunit alpha Calcium/calmodulin-dependent protein kinase type IIA CaM kinase II alpha chain CaM kinase II alpha subunit CaM kinase II subunit alpha CaMK II alpha subunit CaMK-II subunit alpha Camk2a CAMKA CaMKII CaMKIINalpha EC 2.7.11.17 KCC2A_HUMAN KIAA0968 MGC123320 MGC139375 MGC155201 mKIAA0968 PK2CDD PKCCD R74975 zgc:112538 zgc:123320 Calcium / calmodulin dependent protein kinase 2 delta Calcium / calmodulin dependent protein kinase II delta calcium/calmodulin-dependent protein kinase (CaM kinase) II delta calcium/calmodulin-dependent protein kinase type II delta chain Calcium/calmodulin-dependent protein kinase type II subunit delta CAM kinase 2 delta CAM kinase II delta CaM kinase II delta subunit CaM kinase II subunit delta CaM-kinase II delta chain CAMK 2d CaMK-II delta subunit CaMK-II subunit delta CAMK2D CAMKD CAMKI KCC2D_HUMAN RATCAMKI |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Sarcolemma. Sarcoplasmic reticulum membrane. |
| SwissProt ID | Q9UQM7/Q13557 |
|---|
ELISA
Coat the plate with 100ng immunizing antigen. The phospho-specific antibody,pbs only can specifically recognize phosphorylated peptide and enable quantitative detection over a wide dynamic range. Non-phosphorylated peptide shows no cross-reactivity, and the background signal remains low, indicating that the phospho-specific antibody,pbs only possesses high specificity and an excellent signal-to-noise ratio. Black line: phospho-peptide; Rad line: non-phospho peptide
DB
Dot blot analysis was performed using Anti-Phospho-CaMKII alpha/delta (Thr286) 277-288 antibody to detect two peptide samples: lane 1 contains the phospho-peptide , and lane 2 contains the non-phospho-peptide. Transfer the proteins onto a NC membrane (OM790001), and block it with TBST (OM750016) containing 5% BSA(OM297470) for 1 hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it at room temperature for 2 hours. Wash the membrane 3 times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:10000 and incubate for 1 hour. Wash the membrane 3 times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence. Exposure time: 30 s.| Application Notes | DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
