All products Antibodies Rabbit anti-SMAD5 polyclonal antibody - middle region

Rabbit anti-SMAD5 polyclonal antibody - middle region

Catalog Number：OM105594

  • Lane 1: 5ug of transfected 293T lysate (SMAD1)
    Lane 1: 5ug of transfected 293T lysate (SMAD2)
    Lane 1: 5ug of transfected 293T lysate (SMAD3)
    Lane 1: 5ug of transfected 293T lysate (SMAD4)
    Lane 1: 5ug of transfected 293T lysate (SMAD5)
    Lane 1: 5ug of transfected 293T lysate (SMAD6)
    Lane 1: 5ug of transfected 293T lysate (SMAD7)
    Lane 1: 5ug of transfected 293T lysate (SMAD8)
    Lane 1: 5ug of transfected 293T lysate (GFP)
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Goat anti-Rabbit IgG HRP Conjugated
    Secondary Antibody Dilution:
    1:10,000
    Gene Name:
    SMAD5
    Submitted by:
    Amanda Urick, Medical College of Wisconsin

  • 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: HepG2 cell lysate

Product Profile

Product Name Rabbit anti-SMAD5 polyclonal antibody - middle region
Antibody Type Primary Antibodies
Immunogen 
The immunogen for anti-SMAD5 antibody: synthetic peptide directed towards the middle region of human SMAD5

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineDogHorseHumanMouseRatZebra Fish
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SMAD5
Gene Synonyms
DWFC
JV5-1
MADH5
Gene Full Name SMAD family member 5
Gene Summary
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection.
Alternative Names
DWFC
JV5-1
MADH5
Molecular Weight(MW) 51kDa
Sequence 465 amino acids

Database Links

Entrez Gene 4090
SwissProt ID Q99717
Protein Accession NP_005894

Application

  • Immunoblot

    Lane 1: 5ug of transfected 293T lysate (SMAD1)
    Lane 1: 5ug of transfected 293T lysate (SMAD2)
    Lane 1: 5ug of transfected 293T lysate (SMAD3)
    Lane 1: 5ug of transfected 293T lysate (SMAD4)
    Lane 1: 5ug of transfected 293T lysate (SMAD5)
    Lane 1: 5ug of transfected 293T lysate (SMAD6)
    Lane 1: 5ug of transfected 293T lysate (SMAD7)
    Lane 1: 5ug of transfected 293T lysate (SMAD8)
    Lane 1: 5ug of transfected 293T lysate (GFP)
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Goat anti-Rabbit IgG HRP Conjugated
    Secondary Antibody Dilution:
    1:10,000
    Gene Name:
    SMAD5
    Submitted by:
    Amanda Urick, Medical College of Wisconsin

  • Western blot

    0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: HepG2 cell lysate

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM105594
Submit an review for OM105594
  • Catalog Number: OM105594
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: In stock
  • Lead time: 2~3 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.