Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on transfected HEK-293 at: 1:1000 (Lane1: 2ug HEK293lysate, Lane2: 2ug SMAD2 transfected HEK293 lysate).
Product Name | SMAD2 Antibody |
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Antibody Type | Primary Antibodies |
Product description |
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Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD2. |
Clonality | Polyclonal |
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Host Species | Rabbit |
Tested Applications | |
SMAD2 antibody can be used for detection of SMAD2 by ELISA at 1:1562500. SMAD2 antibody can be used for detection of SMAD2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
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Species Reactivity | |
Concentration | 1 mg/ml |
Purification | Protein A |
Gene Symbol | SMAD2 |
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Alternative Names | SMAD2 JV18 MADH2 MADR2 JV18-1 hMAD-2 hSMAD2 |
Molecular Weight(MW) | 51 kDa, 52 kDa, 48 kDa |
Entrez Gene | 4087 |
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Protein Accession | NP_005892 |
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on transfected HEK-293 at: 1:1000 (Lane1: 2ug HEK293lysate, Lane2: 2ug SMAD2 transfected HEK293 lysate).
Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
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Application Notes | SMAD2 antibody can be used for detection of SMAD2 by ELISA at 1:1562500. SMAD2 antibody can be used for detection of SMAD2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
Form | Liquid |
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Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
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