All products Antibodies SMAD4 Antibody

SMAD4 Antibody

Catalog Number：OM291666

  • Antibody used in WB on Human U2OS.

  • Antibody used in WB on Human HepG2 at 1.25 ug/ml.

  • Antibody used in WB on Human U2OS at 1:1000.

Product Profile

Product Name SMAD4 Antibody
Antibody Type Primary Antibodies
Product description 
SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction1) Kantola, A.K., et al., Gene 363, 142-150 (2005).
Immunogen 
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

ELISAWB
SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol SMAD4
Alternative Names
SMAD4
JIP
DPC4
MADH4
MYHRS
Molecular Weight(MW) 60 kDa

Database Links

Entrez Gene 4089
Protein Accession NP_005350

Application

  • Application

    Antibody used in WB on Human U2OS.

  • Application

    Antibody used in WB on Human HepG2 at 1.25 ug/ml.

  • Application

    Antibody used in WB on Human U2OS at 1:1000.

Positive Control 1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:

Additional Information

Form Liquid
Storage Instructions For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM291666
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week

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