All products Antibodies SMAD4 Antibody

SMAD4 Antibody

Catalog Number：OM291669

  • Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Fluorescent confocal image of Hela cell stained with SMAD4 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidi

  • Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Western blot analysis of SMAD4 using rabbit polyclonal SMAD4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

Product Profile

Product Name SMAD4 Antibody
Antibody Type Primary Antibodies
Product description 
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.1) Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004).
Immunogen 
This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-428 amino acids from the C-terminal region of human SMAD4.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIFIHC-PWB
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml

Target Information

Gene Symbol SMAD4
Alternative Names
Mothers against decapentaplegic homolog 4
MAD homolog 4
Mothers against DPP homolog 4
Deletion target in pancreatic carcinoma 4
SMAD family member 4
SMAD 4
Smad4
hSMAD4
SMAD4
DPC4
MADH4
Molecular Weight(MW) 60 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine

Database Links

Entrez Gene 4089
Protein Accession Q13485

Application

  • Application

    Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Fluorescent confocal image of Hela cell stained with SMAD4 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidi

  • Application

    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western blot analysis of SMAD4 using rabbit polyclonal SMAD4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM291669
  • Price: $336.00
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