All products Antibodies SMAD4 Antibody

SMAD4 Antibody

Catalog Number：OM291668

  • Confocal immunofluorescent analysis of SMAD4 Antibody (T277) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • Western blot analysis of SMAD4 Antibody (T277) using rabbit polyclonal SMAD4 Antibody (T277) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

Product Profile

Product Name SMAD4 Antibody
Antibody Type Primary Antibodies
Product description 
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.1) Sekiya, T., et al., Biochem. Biophys. Res. Commun. 320(3):680-684 (2004).
Immunogen 
This SMAD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from human SMAD4.

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFWB
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol SMAD4
Alternative Names
Mothers against decapentaplegic homolog 4
MAD homolog 4
Mothers against DPP homolog 4
Deletion target in pancreatic carcinoma 4
SMAD family member 4
SMAD 4
Smad4
hSMAD4
SMAD4
DPC4
MADH4
Molecular Weight(MW) 60 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Mouse

Database Links

Entrez Gene 4089
Protein Accession Q13485

Application

  • Application

    Confocal immunofluorescent analysis of SMAD4 Antibody (T277) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • Application

    Western blot analysis of SMAD4 Antibody (T277) using rabbit polyclonal SMAD4 Antibody (T277) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

Submit an review for OM291668
Submit an review for OM291668
  • Catalog Number: OM291668
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: Out of stock
  • Lead time: 4~6 Week

  • Quantity:
    - +
  • Shipping to (Please select)
Add to cart
Add to Wishlist
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.
PRODUCT CATEGORIES
Primary antibodies Secondary antibodies Tags antibodies Flow cytometry antibodies
SUPPORT
Catalogs Newsletter Order Online Buying FAQs Distributor
COMPANY
Profile Strategy Careers
CONTACT
Contact us

2013 © Omnimabs , All Rights Reserved.