Application
Western blot analysis of TRIM41 expression in Jurkat whole cell lysate.| Product Name | U11/U12 snRNP 35K Antibody (G-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Small nuclear ribonucleoproteins, also known as snRNPs, combine with other proteins to form spliceosomes, a complex that catalyzes pre-mRNA splicing. There are two types of spliceosomes: U2 and U12. The U2-type spliceosome is found in all eukaryotes and excises U2-type introns, which account for the majority of pre-mRNA introns. The U12-type spliceosome removes U12-type introns, which comprise less than 1% of all human introns. The U12-type spliceosome is comprised of the U11 and U12 snRNPs as well as the U4/U6.U5 tri-snRNP. U11 and U12 bind as a U11/U12 di-snRNP complex, which recognizes the 5’ splice site of the pre-mRNA during the first steps of U12-type spliceosome formation. U11/U12 snRNPs contain several proteins, including seven that are unique to the U11/U12snRNP: 65K, 59K, 48K, 35K, 31K, 25K and 20K. U11/U12 snRNP 35K is a 246 amino acid protein localized to the nucleus, and it contains a motif known to mediate RNA binding. Two named isoforms of this protein exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of U11/U12 snRNP 35K of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against U11/U12 snRNP 35K of human origin |
|---|---|
| Tissue Specificity | raised against U11/U12 snRNP 35K of human origin |
| Entrez Gene | 11066 |
|---|
Application
Western blot analysis of TRIM41 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of U11/U12 snRNP 35K of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM204200