Application
IHC-Fr analysis of human tonsil tissue using CD3 antibody [UCHT1] (Low endotoxin, azide free).Application
Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control. Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes| Product Name | CD3 antibody (Azide free) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [UCHT1] to CD3The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | UCHT1 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanChimpanzee |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | T3E TCRE CD3e molecule epsilon (CD3-TCR complex) CD3E |
|---|---|
| Target | CD3 |
| Tissue Specificity | This antibody recognizes the human T-cell surface glycoprotein CD3 epsilon chain. Clone UCHT1 binds to a region in the ectodomain of human CD3ε and has been shown to bind to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interfac |
Application
IHC-Fr analysis of human tonsil tissue using CD3 antibody [UCHT1] (Low endotoxin, azide free).
Application
Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control. Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 . All experiments performed on human Peripheral blood lymphocytes| Application Notes | Flow Cytometry: 1/50 - 1/100 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline |
