Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] diluted at 1:250. Red: GFAP, stained by GFAP antibody diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:500.Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [GT7711] dilution: 1:1000Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:3000.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:3000.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.| Product Name | Glutamine synthetase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT7711] to Glutamine synthetase (glutamate-ammonia ligase)Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM] |
| Immunogen | Full length human Glutamine synthetase Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT7711 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (85%), Dog (97%), Pig (96%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | glutamate-ammonia ligase GLNS GS PIG59 PIG43 GLUL |
|---|---|
| Target | Glutamine synthetase |
| Molecular Weight(MW) | 42 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] diluted at 1:250. Red: GFAP, stained by GFAP antibody diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:500.
Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT7711] diluted at 1:250. Red: PKC alpha protein stained by PKC alpha antibody [GT1876] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Glutamine synthetase antibody [GT7711] detects Glutamine synthetase protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 10 % SDS-PAGE Glutamine synthetase antibody [GT7711] dilution: 1:1000
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:3000.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT7711] diluted at 1:3000.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , rat brain , mouse eye |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
