| Product Name | Lamin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [JOL4] to Lamin ANuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A type lamins coincides with cell differentiation and as A type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A type lamins. |
| Immunogen | Recombinant human lamin A |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | JOL4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-FIPWB |
| For WB: Use at 1/50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | k66d12 LMN1 70 kDa lamin cb948 CDCD1 CDDC CMD1A CMT2B1 EMD2 FPL |
|---|---|
| Target | Lamin A |
| Tissue Specificity | The antibody reacts with both recombinant and native lamin A in humans, binding to an epitope between amino acids 572-646 |
| Cellular Localization | Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleaveage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature lamin-A/C, which can then be inserted into the nuclear lamina. EMD is required for proper localiza |
| Application Notes | For WB: Use at 1/50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. For extended storage, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 510% foetal calf serum |
