Application
Western blot analysis using anti-MLL monoclonal antiobdy against truncated MLL recombinant protein(1) and truncated GFP-MLL(aa3714-3969) transfected COS-7 cell lysate(2).Application
Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and esophagus cancer(right), showing nuclear weak staining with DAB staining using anti-MLL antibody.| Product Name | MLL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [10F8D7] to MLLThis gene encodes a transcriptional coactivator that plays an essential role in regulating gene expression during early development and hematopoiesis. The encoded protein contains multiple conserved functional domains. One of these domains, the SET domain, is responsible for its histone H3 lysine 4 (H3K4) methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. This protein is processed by the enzyme Taspase 1 into two fragments, MLL-C and MLL-N. These fragments reassociate and further assemble into different multiprotein complexes that regulate the transcription of specific target genes, including many of the HOX genes. Multiple chromosomal translocations involving this gene are the cause of certain acute lymphoid leukemias and acute myeloid leukemias. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2010] |
| Immunogen | The immunogen corresponding to a region within amino acids 3751 and 3968 of MLL expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 10F8D7 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HRX MLL1A WDSTS CXXC7 TRX1 ALL-1 MLL/GAS7 HTRX1 KMT2A TET1-MLL |
|---|---|
| Target | MLL |
| Cellular Localization | Localizes to a diffuse nuclear pattern when not associated with MLL cleavage product N320. |
Application
Western blot analysis using anti-MLL monoclonal antiobdy against truncated MLL recombinant protein(1) and truncated GFP-MLL(aa3714-3969) transfected COS-7 cell lysate(2).
Application
Immunohistochemical analysis of paraffin-embedded human lung cancer(left) and esophagus cancer(right), showing nuclear weak staining with DAB staining using anti-MLL antibody.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
