All products Antibodies Rabbit anti-SMAD4 polyclonal antibody - middle region

Rabbit anti-SMAD4 polyclonal antibody - middle region

Catalog Number：OM105590

  • SMAD4 antibody - middle region validated by WB using U2OS nuclear extracts

  • Sample Type: Human nuclear cell extracts (30ug)
    Primary Dilution: 1:1000
    Secondary Antibody: anti-Rabbit HRP
    Secondary Dilution: 1:20000
    Image Submitted By:  Katarina Luciakova
    Cancer Research Institute See Customer Feedback tab for detailed information.

  • 1.25ug/ml
    ELISA Titer: 1:312500
    Positive Control: HepG2 cell lysate

Product Profile

Product Name Rabbit anti-SMAD4 polyclonal antibody - middle region
Antibody Type Primary Antibodies
Immunogen 
The immunogen for anti-SMAD4 antibody: synthetic peptide directed towards the middle region of human SMAD4

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineGoatGuinea PigHorseHumanMousePigRat
Concentration 1 mg/ml
Purification Protein A

Target Information

Gene Symbol SMAD4
Gene Synonyms
JIP
DPC4
MADH4
MYHRS
Gene Full Name SMAD family member 4
Gene Summary
SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
Alternative Names
JIP
DPC4
MADH4
MYHRS
Molecular Weight(MW) 60kDa
Sequence 552 amino acids

Database Links

Entrez Gene 4089
SwissProt ID Q13485
Protein Accession NP_005350

Application

  • Western blot

    SMAD4 antibody - middle region validated by WB using U2OS nuclear extracts

  • Western blot

    Sample Type: Human nuclear cell extracts (30ug)
    Primary Dilution: 1:1000
    Secondary Antibody: anti-Rabbit HRP
    Secondary Dilution: 1:20000
    Image Submitted By:  Katarina Luciakova
    Cancer Research Institute See Customer Feedback tab for detailed information.

  • Western blot

    1.25ug/ml
    ELISA Titer: 1:312500
    Positive Control: HepG2 cell lysate

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM105590
  • Price: $336.00
  • Discount Price
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  • Store: In stock
  • Lead time: 2~3 Week

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